Nervous Necrosis Virus (NNV) -Susceptibility and -Productivity of Newly Established Cell Lines Derived from Sevenband Grouper <i>Hyporthodus septemfasciatus</i>
نویسندگان
چکیده
Multiple cell lines permissive to nervous necrosis virus (NNV) infection may be necessary for identifying receptor binding sites of viral surface protrusions. We have established new lines, SeGE-12, SeGE-22, SeGF and SeGB, derived from eyed-egg embryos, fin brain sevenband grouper. The optimum growth temperatures these were all around 30°C. temperature range SeGE-12 SeGE-22 was 25°C–32°C, whereas SeGB it 15°C–32°C. These exhibited no cytopathic effect (CPE) due NNV infection, but released extracellular at 102.8 104.7 TCID50/mL after 14 days incubation.
منابع مشابه
Complete Genome Sequence of Nervous Necrosis Virus Isolated from Sevenband Grouper (Epinephelus septemfasciatus) in South Korea
The draft genome sequence of the nervous necrosis virus (NNV) SGYeosu08, isolated from sevenband grouper (Epinephelus septemfasciatus) in Yeosu, South Korea, was cloned and analyzed. The full-length RNA1 was a 3,103-nucleotide-encoding region of RNA-dependent RNA polymerase, and the RNA2 encoding a coat protein was 1,433 nucleotides in length. This genome sequence might be useful in the develop...
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ژورنال
عنوان ژورنال: Fish Pathology
سال: 2021
ISSN: ['0388-788X', '1881-7335']
DOI: https://doi.org/10.3147/jsfp.56.22